Tuesday, May 19, 2026

Even after adopting cattle, early herders kept hunting and gathering

 

Eastern Africa’s earliest livestock herders continued fishing, hunting and gathering for centuries after livestock were first brought to the region. The strategy may have helped them adapt to a harsh, changing climate.

The first pastoralists in eastern Africa didn’t suddenly switch to a diet centered only on cows, sheep and goats. Instead, they kept eating a wide mix of foods—fish, wild animals and plants—alongside livestock for at least 1,000 years.

That’s the key finding from new University of British Columbia-led research published in the journal Proceedings of the National Academy of Sciences. It challenges a long‑held idea that once people begin producing their own food, they quickly narrow down what they eat.

“These early herders didn’t put all their eggs in one basket,” said geochemist Dr. Kendra Chritz, lead author and assistant professor at the University of British Columbia’s Department of Earth, Ocean and Atmospheric Sciences. “They were keeping livestock, but they were also still fishing and hunting and gathering. Their diets were incredibly varied.”

Instead, early herders around Lake Turkana, in what is now northern Kenya, hedged their bets. Even with domesticated animals at hand, they still fished the lake, hunted wild game and gathered plant-based foods.

This matters because food choices shape health, culture and survival. For most of human history, people lived in environments that changed fast. Understanding how our ancestors coped can shed light on how we can adapt to climate stress today. It also reminds us that human diets were never one-size-fits-all. Variety was the norm, not the exception.

To figure this out, the team analyzed chemical clues locked in ancient human teeth. These clues come from stable isotopes—natural markers in teeth that reflect what a person ate while the tooth was forming. In simple terms, teeth keep a long-term record of diet, like a fossilized food diary.

The researchers studied remains from more than 100 people who lived between about 9,500 and 200 years ago in Kenya and Tanzania. They compared fishers and foragers, early herders and later herders who lived after livestock had become central to daily life.

The biggest surprise came from the earliest herders, who lived around 5,000 years ago. Their tooth records showed huge variation from person to person. Some ate foods linked to grass-eating animals, such as cattle. Others relied much more on fish or wild animals. Many did both.

“This level of diversity looks a lot like what we see among hunter-gatherers,” said Dr. Chritz.

“The isotopic record is fascinating because it can reveal individual to individual variation, even among fisher foragers who lived at a single site,” said study co-author Dr. Elisabeth Hildebrand, an associate professor of anthropology at Stony Brook University, who has co-directed excavations at several sites on the west side of Lake Turkana. “This kind of information goes beyond what one can discern from the animal bones left at a site after human consumption.” 

Previous populations of fisher-foragers who lived in the region during a moist climate interval known as the African Humid Period also showed striking dietary variation.

“It’s clear that fisher-foragers followed dietary strategies that were situationally specific, or even personalized,” said Dr. Hildebrand. “And the first pastoralists maintained this very individualized approach, even as they began constructing communal cemeteries that involved large social networks connecting hundreds of people.”

The team also looked at residues left behind in ancient ceramic cooking pots. These fatty traces—basically food stains locked into clay—reveal what was cooked long ago. Some pots from early herders contained fats from animals, but only rarely showed signs of dairy foods like milk. That suggests livestock were important, but not the mainstay of meals.

“Ceramic residue data are an incredible archive—we can see not only what people were cooking, but also the general types of plants, for example, that animals were eating,” explained co-author Dr. Katherine Grillo, an associate professor of anthropology at the University of Florida. “Now that we have isotopic data from ancient human teeth as well, we have a remarkably holistic body of evidence for both ancient environmental changes and the complicated cultural decisions people were making, perhaps in response, about food.”

The arrival of herding around Lake Turkana coincided with major environmental upheaval. The region was drying fast, causing lake levels to drop. Grasslands were slow to spread. Relying only on animals in that unstable setting may have been risky.

“Livestock are valuable, but they’re also vulnerable,” said Dr. Chritz. “If rainfall is unpredictable and pasture is scarce, having multiple food options can make the difference between getting by and going hungry.”

Only later—more than a thousand years after herding began—did diets narrow. Herders in southern Kenya and northern Tanzania eventually relied much more heavily on livestock products. Their tooth records show less variation, suggesting more specialized diets linked to more stable environments.

“This research is a testament to what becomes possible when Kenyan institutions are genuine partners in global science—not just sites of data collection, but active contributors to knowledge production,” said Dr. Emmanuel Ndiema, co-author on the study and head of Earth Sciences at the National Museums of Kenya. “The findings illuminate the ingenuity and resilience of the people who shaped this landscape thousands of years ago, and we are proud that Kenya’s heritage is at the centre of this story.”

Neanderthals gathered shellfish using the same strategies as modern humans


Neanderthal populations in southern Europe collected shellfish throughout the year, with a marked preference for the colder months, according to a new international study led by researchers from the Institute of Environmental Science and Technology at the Universitat Autònoma de Barcelona (ICTA-UAB), the IsoTOPIK Lab at the University of Burgos (UBU), and the Instituto Internacional de Investigaciones Prehistóricas de Cantabria at the University of Cantabria (UC).

The research, recently published in the journal Proceedings of the National Academy of Sciences (PNAS), shows that 115,000 years ago Neanderthal groups from Los Aviones Cave (Cartagena, Region of Murcia, Spain) were already consuming molluscs following a clearly seasonal pattern, particularly during the colder months of the year, from November to April.

For decades, the ability of Neanderthal populations to adapt to coastal environments and exploit marine resources in an organised manner has been the subject of intense debate in archaeology and human evolution. Traditionally, the regular consumption of shellfish and seasonal planning were considered traits exclusive to our species, Homo sapiens. However, this recent finding challenges that paradigm.

The study analysed marine mollusc remains (including small gastropods and limpets) recovered from Los Aviones Cave at an unprecedented resolution. The results show that these populations not only collected shellfish sporadically but also possessed a deep understanding of marine ecological cycles, anticipating by thousands of years behaviours later documented in modern humans from the region.

But how is it possible to determine the season in which a mollusc was consumed thousands of years ago? The key lies in the oxygen isotopic signal preserved in the carbonate of their shells, as the incorporation of heavier or lighter oxygen isotopes depends primarily on seawater temperature. “By reconstructing variation during shell growth, these values act as a prehistoric thermometer. This makes it possible to infer temperature changes as well as the exact time of year when a mollusc was collected, revealing new details about seasonal consumption patterns,” explains Asier García-Escárzaga, lead author of the study.

The results represent a milestone, as they are the first obtained for such early stages of human evolution. “They consumed marine resources throughout the year, but with a very clear preference for winter and autumn months. This pattern, very similar to that developed by more recent populations of modern humans in Europe and other regions, cannot be coincidental,” García-Escárzaga explains.

Winter collection coincides with periods when certain mollusc species have higher meat yield and improved sensory qualities (flavour and texture) due to their reproductive cycles. In addition, Neanderthal populations may have avoided collecting shellfish in summer to minimise health risks, such as the proliferation of toxic algae (red tides) or the rapid decomposition of shellfish due to heat, demonstrating a conscious and safe management of marine resources.

These findings suggest that Neanderthals and modern humans may have been more similar than previously thought. The study highlights that this behaviour reflects a diversified diet incorporating high-quality marine proteins (rich in Omega-3 and zinc), which are essential for brain development and reproductive health. “What we see at Los Aviones is a fully modern subsistence strategy,” the authors state. This discovery reinforces the idea that Neanderthals possessed cognitive, social and economic capacities comparable to our own, establishing the Iberian Peninsula as a key region for understanding the complexity of our closest ancestors.

 

Saturday, May 16, 2026

Humans are the only primates with a population-wide hand preference

A new Oxford-led study, published in PLOS Biology, traces it back to bipedalism and brain expansion.


It is one of the strangest puzzles in human evolution. About 90% of people across every human culture favour their right hand - with no other primate species showing a population-level preference on this scale. Despite decades of research into the brains, genes and development behind handedness, why humans ended up so overwhelmingly right-handed has remained an evolutionary enigma.

Now, new research led by the University of Oxford, published in PLOS Biology, suggests the answer comes down to two defining features of human evolution - walking on two legs, and the dramatic expansion of the human brain.

The study, by Dr Thomas A. Püschel and Rachel M. Hurwitz at Oxford’s School of Anthropology and Museum Ethnography, with Professor Chris Venditti at the University of Reading, brought together data on 2,025 individuals across 41 species of monkeys and apes. Using Bayesian modelling that accounts for evolutionary relationships between species, the team tested the major existing hypotheses for why handedness evolved: including tool use, diet, habitat, body mass, social organisation, brain size and locomotion.

Humans sat conspicuously outside the pattern that explained every other primate, but when the researchers added two factors into the model - brain size and the relative length of our arms versus our legs (a standard anatomical marker of bipedal locomotion) - that exceptional status disappeared. In other words, once you account for upright walking and a large brain, humans stop looking like an evolutionary anomaly.

Using the same models, the team was also able to estimate likely handedness in extinct human ancestors. The picture that emerges is a gradient; early hominins such as Ardipithecus and Australopithecus probably had only mild rightward preferences, broadly similar to modern great apes. With the appearance of the genus Homo, the bias strengthens markedly - through Homo ergaster, Homo erectus and Neanderthals - reaching its modern extreme in Homo sapiens.

There is one striking exception: Homo floresiensis, the small-brained “hobbit” species from Indonesia, shows a much weaker predicted preference. The researchers suggest this fits the wider pattern: floresiensis had a small brain and a body adapted to a mix of upright walking and climbing, rather than full bipedalism.

The findings point to a two-stage story. Walking upright came first, freeing the hands from the work of locomotion and creating new selective pressure for fine, lateralised manual behaviours. Larger brains came later, and as they grew and reorganised, the rightward bias hardened into the near-universal pattern seen today.

Dr Thomas A. Püschel, Wendy James Associate Professor in Evolutionary Anthropology at the University of Oxford, said: ‘This is the first study to test several of the major hypotheses for human handedness in a single framework. Our results suggest it is probably tied to some of the key features that make us human, especially walking upright and the evolution of larger brains. By looking across many primate species, we can begin to understand which aspects of handedness are ancient and shared, and which are uniquely human.’

The study leaves open questions for future research, including the role of cumulative human culture in stabilising right-handedness, why left-handedness has persisted at all, and whether similar patterns of limb preference seen in animals such as parrots and kangaroos point to a deeper, convergent story across the wider animal kingdom.


‘Bipedalism and brain expansion explain human handedness’, Püschel, T. A., Hurwitz, R. M., Venditti, C., PLOS Biology(2026).

URL: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003771

Friday, May 15, 2026

Peruvian hairless dogs discovered at ancient site


Bone specimens and a mummified dog provide evidence of their coexistence with humans.

Peer-Reviewed Publication

Dartmouth College

A puppy skull from Castillo de Huarmey in Peru. 

image: 

A puppy skull from Castillo de Huarmey in Peru. This individual was buried with the “Master Basketmaker.” 

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Credit: Photo by Weronika Tomczyk.

Peruvian hairless dogs—a medium-sized elegant Indigenous breed with pointy ears—a variation of which is today known as a Peruvian inca orchid breed by the American Kennel Club, are widely represented in ancient Andean coastal pottery. Celebrated as a national symbol, they were declared part of Peru's cultural heritage in 2000.

A new study published in the Journal of Anthropological Archaeology provides the first physical evidence of Peruvian hairless dogs from the only Wari Empire site found to date, on the coast of northern Peru, known as Castillo de Huarmey.

Situated about 190 miles north of Lima, the 110-acre site is a Wari administrative and funerary complex that existed between 600 and 1050 CE, 400 years before the Inca rose to power.

The site is famous for the discovery of the first undisturbed elite tomb in 2012 with 58 high-status females and 1,300 artifacts and six human sacrifices buried, and the side burials of elite craftsmen with gold and silver and bronze tools in 2022, yielding extraordinary finds and information about the Wari Empire's provincial management.

"Our findings indicate that humans and dogs coexisted at this Wari site, but reconstructing their bond is challenging, as past emotions are difficult to capture through archaeological methods," says first author, Weronika Tomczyk, a research associate in the Department of Anthropology and postdoctoral fellow in the Ecology, Evolution, Environment and Society Program at Dartmouth. “The goal of our study was to not only present data on Castillo de Huarmey’s dog burials, but also emphasize that people in the past had often contradictory relationships with the animals, just as they do today: a pet for one group of people could at the same time be considered a pest by their closest neighbors."

The site’s vicinity is one of the driest, hyper-arid deserts along the Pacific coast, which helped preserve organic materials and artifacts, such as hair, bone, decorated pottery, pigment, clothes, and leather.

One of the many artifacts found was a ceramic vessel in the shape of a seated, anthropomorphized Peruvian hairless dog holding an object resembling an instrument.

The remains of many South American camelids—most likely domesticated llamas and alpacas, which may have been funerary offerings and refuse—were discovered at the site between 2010 and 2025, and the archaeologists also found dog skeletal remains.

Large portions of Castillo de Huarmey have been heavily looted due to the 1970 earthquake, which caused one of the site's sides to collapse, opening up burial chambers with pottery and artifacts. While the deeper, never-looted layer preserved the Wari context, the upper layers where most of the animal remains have been found were intermixed.

As the archaeologists were exploring the northern part of the main ceremonial area, they found three sets of dog skeletal remains that caught their special attention. The first one was a naturally mummified dog skull with its hairless skin visible and ears still attached, painted with cinnabar—a popular pigment used to adorn deceased in ancient Peru. The team also found a larger dog skull, and a naturally mummified male dog skeleton in a shallow pit with only its lower front limbs missing. Initially, the team thought that the dog may have been buried 50 years ago when the looting had occurred, but Tomczyk noticed that the dog was hairless and all three individuals were missing their first premolars.

"What I noticed is that some of the dog skeletons were missing teeth, and not that they lost them during their lifespan; some teeth, especially first premolars and sometimes last molars, had just never erupted,” said Tomczyk. "And then I found in the literature that the same gene which is responsible for hairlessness in dogs is responsible for a reduced number of teeth, indicating that these were Peruvian hairless dogs."

The researchers used standard zooarchaeological methods to estimate how old the dogs were. They also conducted an isotopic analysis of bone and teeth, which provide information about the life of the animal such as what it ate and drank, and where it came from, based on the isotopes of carbon, nitrogen, oxygen, and strontium. The latter element represents the bioavailable geological signals of the region where an individual lived during a formation of specific sampled tissue, so it can be used to infer a provenance or migration patterns. Then, the researchers compared the data to camelid and human data from the site.

"Isotopic analyses of a dog's tooth give a signal of its life as a puppy because dog teeth erupt so fast, and the bone gives a signal of more or less the last year of its life," said Tomczyk. "Such sampling strategy aimed to provide signals from distinct stages of a dog’s life.”

The research team recovered 341 dog bone specimens that are estimated to have come from a minimum of 19 dogs, as well as the complete mummified male dog. These included the bones of a 6- to 8-week-old puppy that were buried with one of the elite craftsman, nicknamed the "Master Basketmaker," bones of an adult dog that were buried in the site’s palace, and bones of a partial puppy skeleton that were buried with a male guardian recorded as XY, who was likely sacrificed as a tomb guardian.

Most of the dogs were adults when they were buried. Some were buried intentionally while others were just found as refuse. And very few had butchery marks indicating that they were not widely consumed.

In radiocarbon dating the mummified dog, the team determined that the dog is at least 1,200 years old, providing the oldest radiocarbon date from Castillo de Huarmey to date, which has important ramifications for the site's chronology.

The isotopic analysis revealed that most dogs consumed some maize—a staple in the Pre-Hispanic Andes, indicating that their diets were similar to those of humans. Moreover, when tested dogs suspected of hairlessness were puppies, their diet was more similar to the diet of children, whereas when they were adults, their diet became more variable.

"We do not know if the dogs were intentionally fed maize or if perhaps, they were just eating leftovers or trash," said Tomczyk. "Yet, the distinct dietary patterns of hairless puppies provide insights into what may have been organized breeding."

"While we will never know if any of the three dogs were pets or how people treated them, it is evident they received different treatment than other dogs," said Tomczyk.


Journal

 

Historical DNA connects 1.3 million living relatives to 17th-century Maryland settlers

 

and may have identified the colony’s second governor


Analysis of Historic St. Mary’s City highlights the power of combining ancient DNA, archaeology, and genealogy to restore lost identities and track the migrations of America's earliest English colonists and their descendants.


At a Glance:

  • DNA analysis of 49 colonists from 17th-century St. Mary’s City reveals over 1.3 million living genetic relatives of Maryland’s founding settlers.

  • Researchers used DNA to trace the migration of St. Mary’s colonists from Great Britain and Ireland to Maryland, and subsequent migrations of their descendants across the United States, including a wave of migration of Maryland Catholics to Kentucky following the Revolutionary War. 

  • The study uses a novel framework that combines genetic and genealogical information to propose possible identities for previously unknown individuals buried in Historic St. Mary’s City’s Chapel Field cemetery, leading to the possible identification of Maryland’s second governor, Thomas Greene (1609–1651).

As the United States prepares to mark its 250th anniversary, researchers from 23andMe Research Institute, Harvard University, and the Smithsonian Institution have teamed up to study one of the country’s founding settlements: St. Mary’s City, Maryland. 

Established in 1634, St. Mary’s City was the first English settlement in the colony of Maryland. Despite existing written records and the ability of many present-day Americans to trace their ancestry to the historic city, many gaps remain in our knowledge of this early founder population.

Published in Current Biology, the study focuses on the genetic ancestry of 49 colonists who were buried in St. Mary’s City’s Chapel Field cemetery between 1634–1730. 

By studying their DNA, the research team learned about the ancestral origins of this founding population and traced the migration of their descendants across the United States. Using a novel approach that combines genetic connections to research participants in the 23andMe database with self-reported family history information, they even propose possible identities for three previously unknown individuals buried in the cemetery, including Maryland's second governor, Thomas Greene.

“This work highlights the power of ancient DNA analyses to fill in gaps in the historical record,” said co-senior author David Reich, professor of genetics in the Blavatnik Institute at Harvard Medical School and professor of human evolutionary biology in Harvard’s FAS. “While written records are extraordinarily rich, genetic data can still address gaps in that record and yield surprises.” 

Expanding the Calvert Family

"This project has been decades in the making, starting with the excavation of three rare lead coffins from the Brick Chapel," said Douglas Owsley, curator of biological anthropology at the Smithsonian’s National Museum of Natural History. "Years of interdisciplinary research eventually led us to team up with Harvard for DNA analysis, which became the springboard for these incredible new genetic discoveries."

The lead coffins contained the remains of Philip Calvert, his first wife, Anne Wolseley Calvert, and an infant son, who the genetic analysis revealed to be the son of Calvert and his second wife, Jane Sewell. The results of this genetic analysis were released in 2016 as part of a technical report, but are formally published for the first time in this study. 

Philip Calvert, who served as the colony’s fifth governor, was the youngest son of George Calvert, England’s first Baron Baltimore. The prominent Calvert family played a critical role in the founding of the colony of Maryland. The colony was first envisioned by George Calvert as an expansion of the King's realm and a place of religious freedom, especially for persecuted Catholics. The royal charter for the new colony was granted shortly after his death. In 1633, the first settlers set sail for Maryland aboard the ships The Ark and The Dove, led by George’s son Leonard Calvert. The travelers arrived at Maryland’s southern tip in 1634, where they founded St. Mary’s City. While many members of the Calvert family are known to have traveled to St. Mary’s City, only Philip’s burial location has been identified.

When comparing the DNA of Philip Calvert and his wife and son to the 46 other individuals included in this analysis, the research team identified three additional members of the Calvert family. 

“Although additional work is needed to determine exactly how these individuals were related to Philip, this finding is significant given that several members of the extended Calvert family, including Philip’s half-brothers Leonard (1610–1647) and George (1613–1634), died in St. Mary’s during this period,” said Owsley.

The DNA study also identified related individuals belonging to five additional families buried at the site, including one that spanned three generations. “Because mortality was so high in the early days of the colony, finding a multigenerational family was a surprise,” Owsley added. “It’s a discovery that simply wouldn’t have been possible without genetic study.”

DNA helps trace historic migrations

By studying patterns of genetic sharing between the St. Mary’s individuals and over 11.5 million research participants in the 23andMe genetic database, the researchers were able to identify the likely places of origin for many of the colonial city’s earliest inhabitants. The majority of these individuals share the strongest connection with participants from Great Britain, particularly in western England and Wales, while several appear genetically Irish.

Further, by examining patterns of sharing across the United States, they discovered a strong genetic signal that mirrored a documented migration of Catholics from Maryland to Kentucky that primarily occurred between 1780–1820, driven by economic pressures and anti-Catholic bias in Maryland.

"Detecting such a clear genetic signal of this documented historical migration to Kentucky highlights the power of our approach,” said Éadaoin Harney, Senior Scientist, Population Genetics Research and Development at 23andMe Research Institute. 

Possible identification of the colony of Maryland’s second governor, Thomas Greene (1609–1651) 

By focusing on research participants who shared the strongest genetic connections to the St. Mary’s individuals, and inviting them to share details of their family histories, the research team tackled a novel problem: identifying otherwise unknown individuals by harnessing their genetic connections to living people. 

Focusing on the burials of three related individuals identified at the site, researchers collected genealogical information from study participants who shared the strongest genetic connections to these individuals. By searching for overlaps in their family trees and integrating other anthropological information, the research team concluded that the most probable candidates for the identities of these individuals are Governor Thomas Greene, second governor of the colony of Maryland, his first wife Anne, and their son, Leonard. 

“This is the first time that ancient DNA has been used to help identify unknown individuals, without any prior knowledge of who they might have been. And it just so happens that one of those individuals turned out to be one of colonial Maryland’s most prominent figures” said Harney.

“We didn’t go into this study searching for Thomas Greene, but when the genetics team brought this name to me, it was remarkable how well the historical and archaeological records supported this potential identification,” said Henry Miller, Senior Research Fellow at Historic St. Mary’s City. “There is more work to be done to confirm his identity, but this genetic analysis was the key we needed to unlock this finding.”  

“The possibilities that this kind of approach holds for enabling future reidentifications of historical individuals are truly exciting,” said Reich.

Honoring America's 250th Anniversary through DNA

As the United States approaches its 250th anniversary, this research underscores the enduring connections between the nation's earliest European settlers and millions of living Americans. The project relied not just on cutting-edge science, but on the active support of the early Maryland descendant community, including those who trace their lineage to the original Ark and Dove voyage.

"Community collaboration is at the heart of this work," concluded Miller. "The descendant community has championed this research from the beginning, and this genetic analysis allows us to directly link our archaeological findings with the living people who represent that enduring legacy today. It is incredibly meaningful as we reflect on the founding of our country."

Thursday, May 14, 2026

Researchers reveal new clues about H. erectus evolution

 

Scientists from the Institute of Vertebrate Paleontology and Paleoanthropology (IVPP) of the Chinese Academy of Sciences have uncovered new information suggesting a potential connection between Homo erectus and modern humans, while also developing new, less invasive paleoproteomics methods of fossil research.


Homo erectus, or H. erectus, was the first species within the genus Homo to leave Africa, occupying a key position in human evolutionary history. However, due to the lack of molecular evidence from H. erectus, their genetic characteristics, population diversity, and especially their potential connections to modern humans remain unresolved. As a result, the role of H. erectus represents a major mystery and a focal point of debate in human evolution.

Molecular research on H. erectus remains has been limited because ancient human fossils are irreplaceable and a precious cultural heritage. For this reason, traditional destructive sampling methods are unacceptable and have long constrained the progress of relevant molecular research.

Now, however, the research team, led by FU Qiaomei from IVPP in collaboration with multiple institutions, has overcome this bottleneck by employing a micro-destructive sampling approach based on acid etching to recover molecular information from six Homo erectus teeth without damaging their morphology.

The team’s findings were published online in Nature on May 13.

The article was also accompanied by a concurrent commentary in Nature, which highlighted the role of enamel proteins from these six H. erectus teeth from China in providing “new insights into how ancient genetic material was eventually introduced into modern human populations.” 

The researchers identified two mutations from the fossil teeth, dating back to at least 400,000 years ago, from three different sites—Zhoukoudian (Peking Man), Hexian, and Sunjiadong. The mutations suggest genetic links between East Asian H. erectus and Denisovans, which themselves are linked to modern humans.

The first is the previously unknown AMBN-A253G mutation, which was identified as a potential molecular marker associated with these H. erectus populations. It provides the first evidence that H. erectus specimens from these three sites belonged to the same evolutionary population.

The second is the AMBN-M273V variant, previously thought to be specific to Denisovans. However, this study reveals that this variant is not unique to Denisovans but is shared by these H. erectus populations.

According to the researchers, the second variant may have entered the Denisovan lineage through admixture and was subsequently passed to some modern human populations (in Southeast Asia and Oceania) via Denisovan introgression. This provides the first insights into a possible connection between East Asian H. erectus (such as those from Zhoukoudian) and Denisovans, as well as the potential deep genetic links to some present-day modern humans.

Additionally, the study establishes a suite of new experimental and computational methodologies, including a sex determination method for ancient hominins based on the male-specific enamel protein AMELY, a cross-validation approach using tandem mass spectrometry and multiple data analysis pipelines, and DNA analysis methods linked to specific amino acid variants. Together, these tools provide a new framework for systematic paleoproteomics research.

Neanderthal dentists used stone drills to treat cavities nearly 60,000 years ago

 

A tooth from a Russian cave provides the oldest evidence of complex dental care

Peer-Reviewed Publication

PLOS

Earliest evidence for invasive mitigation of dental caries by Neanderthals 

image: 

Chagyrskaya 64 molar tooth and its macro-features: General view of the tooth in five projections.

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Credit: Zubova et al., 2026, PLOS One, CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/)

Neanderthals had the know-how to identify a tooth infection and the motor skills to drill out the damage, according to a study published May 13, 2026 in the open-access journal PLOS One by Alisa Zubova of Peter the Great Museum of Anthropology and Ethnography, Russian Academy of Sciences (Kunstkamera), St. Petersburg, and colleagues.

Archaeological discoveries have shown that Neanderthals used toothpicks to remove food from their teeth and might also have used medicinal plants, but the extent of their medical capabilities is unclear. In this study, Zubova and colleagues describe a Neanderthal tooth which received physical alterations to treat infection.

This tooth is a single molar from Chagyrskaya Cave in Russia, around 59,000 years old. In the center of the tooth is a deep hole extending into the pulp cavity. The researchers conducted experiments on three modern human teeth to demonstrate that a hole of the same shape and same patterns of microscopic grooves can be created by drilling into the tooth with a stone point similar to tools that have been found within Chagyrskaya Cave. The hole in this damaged molar, as well as toothpick grooves along the side of the tooth, is an example of a caries lesion in the same population, which is rare among Neanderthals.

This procedure would have hurt, but it would also have ultimately alleviated the pain of a tooth infection by removing the damaged part of the tooth. These modifications provide evidence that Neanderthals had the capacity to identify the source of pain, to determine how to treat it, to apply the manual dexterity needed for an efficient operation, and to endure painful treatment to alleviate future discomfort. This is the first time such behavior has been demonstrated outside of Homo sapiens, and it is the oldest example of such behavior by more than 40,000 years.

The authors add: “This finding currently represents the world's oldest evidence of successful dental treatment. The damage documented on the Neanderthal tooth from Chagyrskaya Cave in Siberia points not only to intentional pulp removal but also to antemortem wear - wear that could only have developed if the individual kept using the tooth while alive. We also identified areas of demineralization where remnants of carious damage were preserved, further indicating that the concavity in the tooth was associated with treatment.”

Alisa Zubova adds: "We were intrigued by the unusual shape of the concavity on the tooth's chewing surface. It differed from the normal morphology of the pulp chamber and did not match the typical pattern of carious lesions seen in Homo sapiens. Moreover, distinctly visible scratches suggested that the concavity was not the result of natural damage but of intentional actions."

"Computed microtomography revealed changes in dentin mineralization consistent with severe caries. Human manipulation of carious lesions has already been documented for the Upper Paleolithic, Mesolithic, and later periods. We therefore hypothesized that the damage we observed could also represent traces of such medical intervention - but from a significantly earlier period."

Lydia Zotkina adds:To interpret the concavity on the occlusal surface of the tooth, we conducted experimental manual drilling on a series of specimens: a modern human tooth and two Homo sapiens teeth from a Holocene archaeological collection of uncertain temporal and cultural provenance. Comparison of the microscopic traces on the original Neanderthal specimen with those produced experimentally revealed a clear match. The findings demonstrate that drilling a carious lesion using a sharp, thin stone tool is entirely effective, permitting the rapid removal of damaged dental tissue.”

Ksenia Kolobova adds: “Neanderthals arrived in this region 70–60 thousand years ago during a migration from Central and Eastern Europe and inhabited it until at least 40–45 thousand years ago. Altai became a new and suitable home for them thanks to its biological diversity, climate similar to that of Europe, abundant raw materials for stone tool production, and their usual prey - wild bison and horses. Analysis of stone tool industries and paleogenetic studies have shown that the Neanderthals from Chagyrskaya Cave are very closely related to the bearers of the so-called Micoquian industry, who also lived in the Caucasus and Crimea.”

 

The freely available article in PLOS One: https://plos.io/429tPn